Gender mainstreaming is a transversal dimension of the policy programming and implementation of all Structural Funds. The study outlines to what extent the gender mainstreaming has been taken into account in the 2000-2006 Structural Funds programming. Policy fields (and phases) where progress has been made and policy fields (and phases) where progress is still slow are highlighted. Major findings show that better results are acheived upstream the policy process, rather than the downstream at implementation level. Overall, much has been done to gather the necessary conditions for successful gender mainstreaming over the 2007-2013 period. However, more could be done to translate broad and specific objectives into practice and effective actions.